Neuronal Differentiation of P19 Embryonal Cells in Polyhydroxybutyrate Scaffold as a Model for Nerve Tissue Engineering

M.T. Khorasani, S.A. Mirmohammadi, and S. Irani (Iran)

Keywords

Polyhydroxybutyrate, microtubular scaffold, salt leach,micromoulding, tissue engineering , P19 cell

Abstract

To promote long-term nerve repair, conduits made of biodegradable polymers have greatly attracted attention of tissue engineers. In this study we fabricated PHB scaffolds by two methods and compared their properties. Methods contain micro-molding (using mould), salt leaching (using sodium chloride as salt leach). The properties of fabricated scaffolds were investigated and compared using scanning electron microscopy (SEM) for morphology study, dynamic mechanical thermal analysis (DMTA). Results show that scaffolds made of salt leach method have connectivity between porous and scaffolds made of micro-molding method have better mechanical properties without porous connectivity. For in – vitro evaluation the P19 mouse embryonal carcinoma cell line was used as a suitable model system of neuronal differentiation. P19 cells differentiated into neurons and astrocytes on scaffold as indicated by optical photomicrographs method. Results notice that cells attached and differentiated on the scaffold. In – vitro assay show that it is a suitable model for use as a platform for neural tissue engineering applications.

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