Pulmonary Compliance Imaging in Rodents

T. Guerrero, K. Sanders, R. Castillo, B. Rivera, D. Cody, R. Price, L. Bidaut, and R. Komaki (USA)


Pulmonary compliance, rodents, optical flow, computed tomography


Purpose: To develop a technique for generating a high resolution 3D pulmonary compliance image in a rodent model from CT images. Materials & Methods: A series of three 150 gram Fischer 344 rats were anesthesized, intubated, and mechanically ventilated. A GE flat panel CT scanner (0.15 mm resolution) was utilized for image acquisition. The thorax of each animal was imaged with breath-holds at 2, 6, 10, 14, and 18 cm H2O pressure. A deformable image registration algorithm was applied to each pair of CT images to map corresponding tissue elements. Adjacent pairs of CT images (2 & 6, 6 & 10, 10 & 14, 14 & 18 cm H2O) were utilized to calculate the pulmonary compliance on a voxel by voxel basis. The resulting summed whole lung compliance was compared with that obtained from manual segmentation of the lung volume for each pair. Results: The 3D pulmonary compliance images revealed regional heterogeneity of compliance. The calculated total lung compliance compared favorably with the values obtained using manually segmented lung volumes, with a correlation coefficient of R=0.93. The compliance versus pressure relationship was nonlinear. Conclusions: We have demonstrated a unique method of quantifying regional lung compliance with sub-millimeter spatial resolution in a rat model.

Important Links:

Go Back