Light-Addressable Potentiometric Penicillin Image Sensors using the Self-Assembled Monolayer Immobilizing Method

S.-Y. Lee, S.-W. Jang, D.-H. Kwon, S.-H. Lee, and S.-W. Kang (Korea)


: LAPS, Ta2O5, Penicillin, LabVIEW


In this study, the Light Addressable Potentiometric Sensors (LAPSs) which have devices of Si3N4/SiO2/Si, Ta2O5/SiO2/Si structure were fabricated. In particular, the etched silicon wafers were used to improve sensing capability by using TMAH (tetramethyl ammonium hydroxide) solution. Also, it was investigated the response characteristics to penicillin concentration and detected the 2-D images of 128 x 128 pixels. The penicillinase was immobilized on the devices to hydrolyze the penicillin using SAM (self-assembled monolayer) method. The measuring system was simplified by using LabVIEW software. The result of the enzyme reaction, the sensitivity of the sensor which has Si3N4 surfaces was 60 mV/decade and Ta2O5 surfaces was 74 mV/decade in the range of 0.1 mM ~ 10 mM of the penicillin concentration. Image detecting time by an enzyme reaction between penicillin and penicillinase took about 10 minutes at 128 x 128 pixels in 1 x 1 cm sensing area.

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