Simulating the Continuous Flow Separation Element for DNA Extraction

S. Chen, N. Phan-Thien, X.J. Fan, and B.C. Khoo (Singapore)


Dissipative Particle Dynamics, Separation, DNA, Sphere, Microchannel


The particle suspension flow under external magnetic force in bifurcating micro-channel has been numerically studied using mesoscopic technique, dissipative particle dynamics. The bifurcating micro-channel simulates the continuous flow separation element (CFSE) which is used in the isolation of DNA from whole blood for Polymerase Chain Reaction (PCR). The rotational motion of the spheres is studied in the present study by using the Denavit-Hartenberg representation of linkages in robot arm kinematics. The surrounding fluids are simulated by simple DPD particles. The computational technique is tested by simulating the settling of one sphere in a channel and the rotation of one sphere in shear flow. Migration of the sphere subjecting to the magnetic force is investigated in detail. The conformation evolution of DNA molecule passing through the periodic contraction and diffusion slit is also investigated.

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